The Principle of UV Spectrophotometer is based on the Beer-Lambert Law, which states that absorbance of ultraviolet light is directly proportional to the concentration of the absorbing substance and the path length of the sample cell. Its popularity comes from its versatility, simplicity, and broad applicability, as it can detect nearly every molecule under the right conditions. Being relatively inexpensive and easily implemented, this. Ultraviolet (UV) and visible radiation are a small part of the electromagnetic spectrum, which includes other forms of radiation such as radio, infrared (IR), cosmic, and X rays.